Equipment – INDICASAT

INDICASAT-AIP

It has a series of equipment, which it makes available to private and government companies, as part of its portfolio of services.

Matrix Irradiation Facilitated Desorption and Ionization Mass Spectrometer with dual flight chamber (MALDI-TOF / TOF), ultrafleXtreme, Bruker:

Mass spectrometry is a technique used to measure the mass-charge ratio (m / z) of ions produced from analytes of interest. In this instrument, ionization is facilitated using specific matrices for MALDI (e.g. Sinapinic Acid, α-cyano-4-hydoxycinamic Acid, etc.) that help transfer electronic charges to the analytes when irradiated and excited by a laser. The MALDI technique allows to have a viewing window from which to select from ~ 500 Da to around 200,000 Da.

The ultrafleXtreme equipment has a resolving power of 40,000, which when combined with a fast acquisition speed (2 kHz in TOF mode and 1 kHz in TOF / TOF mode) allows quite flexible workflows that range from protein imaging, analysis of intact proteins, quality control of biological products or oligonucleotides, up to proteomics with MALDI coupled to liquid chromatography. The new TOF / TOF technology allows high efficiency and sensitivity for peptide fragmentation studies (MS / MS) working with small amounts of sample down to the fmol level. The high resolution and speed of data acquisition allows the ultraflextreme to be coupled to a wide variety of complementary applications to the field of research, clinical processes and proteomics.

ElectroSpray Ionization Mass Spectrometer / Atmospheric Pressure Chemical Ionization with Quadruple Analyzer and Time of Flight Camera (ESI / APCI-q-TOF) micrOTOF-QIII, Bruker:

Mass spectrometry is a technique used to measure the mass-charge ratio (m / z) of ions produced from analytes of interest. We currently have two ionization sources for this equipment: an electrospray ionization unit (ESI) and a chemical ionization unit at atmospheric pressure that work through facilitated ionization when the compounds are exposed to an electric field under a drying flow. with inert gas (in our case N2) and exposure to high voltages from a corona needle (in the case of APCI).

A resolution> 16,500 FWHM and an accuracy with an error of less than 2 ppm makes the micrOTOF-QIII one of the most versatile devices in its class. Its resolving power and accuracy in combination with the isotopic pattern comparison software application greatly helps to reduce ambiguity in determining the molecular formula of ions of interest as well as the separation of specific chromatograms of ions of interest with a window less than 2mDa even in experiments that require fast data processing by coupling the mass spectrometer to any HPLC, UHPLC, CE or even GC. Molecular formula verification, De Novo molecular formula formulation, multi-compound analysis, biomarker discovery, and intact protein analysis are just a few of the few applications that high-throughput TOF-MS systems cover.

1290 Infinity Ultra High Efficiency Liquid Chromatograph (UHPLC), Agilent Technologies:

Liquid chromatography is an essential tool in any laboratory associated with chemistry since it allows the separation of complex samples of compounds, taking advantage of the “small” chemical differences between compounds. In the mass laboratory, liquid chromatography is used in LC-MS experiments that couples both systems to separate complex mixtures before being detected by the mass spectrometer and thus increase the viewing window of the available compounds.

The 1290 Infinity is one of the highest performance and most versatile machines in the UHPLC market. Allows a wide variety of applications: experiments with UHPLC methods that can handle pressures up to 1200 bar, adaptation to some HPLC methods, method transfer from HPLC to UHPLC, development of separation methods, multi-compound analysis, coupling to spectrometers mass (LC-MS) and among others.

Fourier Transform Infrared (FTIR) spectrometer ALPHA, Bruker:

Fourier transform infrared spectroscopy (FTIR) is a technique used to obtain an infrared spectrum of absorption, emission, photoconductivity or Raman scattering of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high-resolution spectral data over a wide spectral range.

The ALPHA offers an optimal design for almost every sample and is therefore largely suitable for a wide variety of applications. One of the most important applications of ALPHA is quality control in various industries. Here FTIR spectroscopic analysis is predominantly used to verify the identity and specifications of raw materials and products. In addition, the ALPHA allows the identification of unknown samples. This is particularly valuable for effective failure analysis, for example when examining contamination that caused a product defect. In criminology, the possibility of determining unknown substances is applied to identify drugs, explosives and also trace evidence. Medical diagnostics use the ALPHA to analyze, for example, the nature of kidney stones. The availability of liquid cells and various ATR options for the ALPHA allow the realization of quantitative analysis always with the optimal measurement conditions for the respective type of sample.

Gas Chromatographs for Flame Ionization, Electron Capture, Thermal Conductivity, Nitrogen-Phosphorus, and with Mass Detector, Agilent Technologies:

INDICASAT has gas chromatographs with different types of detectors that allow the analysis of different volatile and semi-volatile compounds, or for the analysis of different types of substances after their derivatization.

These equipments can be used in the analysis of food, environmental samples, oil, criminology studies, biomedical research, among others.